Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays

Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays.

Large-scale, single pass sequencing and parallel gene expression analysis using DNA microarrays were employed for the comprehensive investigation of ripening in strawberry fruit. A total of 1701 cDNA clones (comprising 1100 strawberry ESTs and 601 unsequenced cDNAs) obtained from a strawberry (Fragariaxananassa) ripe fruit cDNA library were displayed on microarrays, and used for monitoring conc...

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Multi-substrate flavonol O-glucosyltransferases from strawberry (Fragaria×ananassa) achene and receptacle

In an effort to characterize fruit ripening-related genes functionally, two glucosyltransferases, FaGT6 and FaGT7, were cloned from a strawberry (Fragaria x ananassa) cDNA library and the full-length open reading frames were amplified by rapid amplification of cDNA ends. FaGT6 and FaGT7 were expressed heterologously as fusion proteins in Escherichia coli and target protein was purified using af...

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DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

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DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

متن کامل

DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

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Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays

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